Takii cell baido TM-1 (Takii & Co.) was used as the planting soil (3 L tray –1). ![]() pekinensis) cultivar “Kigokoro85” (Takii & Co.) were sown in a seedling cultivation cell tray (vegetable tray Yanmar Holdings) under greenhouse conditions on Septemand grown for 31 days. The seeds of the napa cabbage ( Brassica rapa var. The present study investigated the root-associated microbes of napa cabbage after top dressing with an additional fertilizer using Chiyoda-kasei and a conventional compound fertilizer. Therefore, Chiyoda-kasei may also have a unique impact on plant-associated microbes over a conventional compound fertilizer. These unique features of Chiyoda-kasei are advantageous for top dressing with an additional fertilization because they support the fast growth of crops with a rapid and balanced nutrient supply to soil. By reacting liquid and gas in its production processes, each of the chemical components in a particle of Chiyoda-kasei is more evenly distributed within its particles than in a general compound fertilizer ( Fig. ![]() Chiyoda-kasei (SUN AGRO) is a unique fertilizer that is expected to have high solubility and rapid diffusibility from the surface of a field into soil, which is most likely due to its porous structure ( Fig. In contrast to a basal fertilizer, which is thoroughly mixed into soil at the beginning of cultivation, an additional fertilizer is often applied to the soil surface (top dressing fertilization). The impact of basal and additional fertilizations on plant-associated microbes has been assessed in the last decade ( Ikeda et al., 2010b, 2011, 2015 Rodríguez-Blanco et al., 2015 Beltran-Garcia et al., 2021 Wang et al., 2022) however, the effects of qualitative differences in additional fertilizations remain unknown. One of the main reasons for this is most likely due to the lack of sufficient information on the interactions between beneficial microbes and diverse environmental factors, including fertilizers and pesticides ( Ikeda et al., 2010a). However, the practical utilization of the functionality of beneficial microbes remains challenging ( Raymaekers et al., 2020). An understanding of the diversity and functionality of environmental microbes is a key factor for constructing a sustainable agricultural system as a green technology ( Bulgari et al., 2019).
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